The detection was made using ECL detection kit (Abcam, ab65623, MA, USA) with 5 min incubation in a dark room. The detection reagent was drained and then, the membrane was exposed to a sheet of diagnostic film in a film cassette for 1 to 30 min .

Anti-Calreticulin Abcam ab22683 IF Anti-HMGB1 Abcam ab18256 IF Anti-LC3 Cell Signaling 2775s WB, IF Anti-ATG5 Abcam ab228668 WB Anti-ATG7 Abcam ab53255 WB Anti-p62 Abcam ab56416 WB Anti-caspase3a Abcam ab2302 WB Anti-caspase3 Abcam ab13847 WB Anti-eIF2a Abcam ab5369 WB Anti-Sigma1 Santa Cruz Sc22948 IF Anti-CD3 (Alexa Fluor® 647-conjugated)

ab13847, Abcam, Cambridge, MA, USA), cleaved caspase3 (No. ab2302, Abcam), caspase Cyt c rabbit antibody, ab18738, caspase-3 rabbit antibody, ab13847, Abcam, USA; β-actin rabbit antibody, bs-0061R, bioss, Beijing; goat anti-rabbit IgG (H+L), cam, ab108319, Cambridge, UK) and caspase-3 levels. (Abcam, ab13847, Cambridge, UK) were assessed im- munohistochemically in other brain hemispheres 15 Nov 2018 ab15580); anti-Caspase3 (1:500, Abcam ab13847). GFAP-positive as- trocytes were not quantified following detachment and injection, be-. 23 Apr 2018 following primary antibodies: NeuN (Millipore, MAB377, 1:300), Epac2 (1:200, Cell Signaling, 43239), caspase-3 (1:200, Abcam, ab13847). Hybridoma Bank) and anti-activated caspase-3 (1:500; Abcam, ab13847).

Abcam ab13847

A8K5M2, D3DP53, P42574, Q96AN1 ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells. Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. Nov 14, 2018 Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

I can confirm that ab13847 tested in IHC-P on adult rat brain in an Abreview. I can recommend to review this on the datasheet for further information. Lane 1: Wild-type HAP1 cell lysate + Staurosporine (1μM for 4h) Lane 2: Wild-type HAP1 cell lysate Lane 3: Caspase-3 knockout HAP1 cell lysate + Staurosporine (1μM for 4h) Lane 4: Caspase-3 knockout HAP1 cell lysate Lanes 1 - 4: Merged signal (red and green).

7 Dec 2018 Each slide was treated with an adequate amount of diluted caspase-3 antibody ( ab13847, 5 μg/mL, Abcam), placed into humidity chambers

2019;129:1115-1128 pubmed publisher Anti Active Caspase3, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Anti Oxdj 1, supplied by Abcam, used in various techniques.

ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells.

Protein Names.

Introduction. The ability of neural stem cell transplantation to restore brain function has been established in pre-clinical models of stroke [, , ] and has recently been tested in a Phase IIa clinical trial (PISCES-II); however, the optimal state of cell maturity for transplantation remains elusive.
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(Abcam, ab13847, Cambridge, UK) were assessed im- munohistochemically in other brain hemispheres 15 Nov 2018 ab15580); anti-Caspase3 (1:500, Abcam ab13847). GFAP-positive as- trocytes were not quantified following detachment and injection, be-.

I can confirm that ab13847 tested in IHC-P on adult rat brain in an Abreview.
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Abcam), GFI1 (ab21061, 1:1,000, Abcam), MMP-9 (ab38898, 1:1,000, Abcam), PCNA (ab92552, 1:1,000, Abcam), caspase-3 (ab13847, 1:500, Abcam),

ab2302, Abcam), caspase Cyt c rabbit antibody, ab18738, caspase-3 rabbit antibody, ab13847, Abcam, USA; β-actin rabbit antibody, bs-0061R, bioss, Beijing; goat anti-rabbit IgG (H+L), cam, ab108319, Cambridge, UK) and caspase-3 levels. (Abcam, ab13847, Cambridge, UK) were assessed im- munohistochemically in other brain hemispheres 15 Nov 2018 ab15580); anti-Caspase3 (1:500, Abcam ab13847). GFAP-positive as- trocytes were not quantified following detachment and injection, be-. 23 Apr 2018 following primary antibodies: NeuN (Millipore, MAB377, 1:300), Epac2 (1:200, Cell Signaling, 43239), caspase-3 (1:200, Abcam, ab13847). Hybridoma Bank) and anti-activated caspase-3 (1:500; Abcam, ab13847).